Array Based Comparative Genomic Hybridization (aCGH) Services using BAC Microarrays for both Human and Mouse are Now Available in the Hartwell Center's Functional Genomics Lab

aCGH services using human and mouse BAC arrays obtained from the Microarray and Genomics Core Facility at Roswell Park Cancer Institute (RPCI), are now available.  BAC array based CGH provides a high resolution, highly efficient means to detect, quantitate and map regions of abnormal copy number (both gains and losses) by comparing the relative efficiency with which test and reference DNA hybridize across the genome.  The human BAC arrays are comprised of 6000 RPCI-11 BAC clones that have either been completely sequenced or are connected to the human sequence via STS or end sequence content, and annotated for gene content.  Included in this set are ~1300 BACs containing known tumor suppressor genes, known microdeletion syndromes, telomeric BACs, oncogenes, and genes associated with the cancer phenotype. The human genomic arrays are produced at 0.5 Megabase resolution and are printed in duplicate on a single slide.  The mouse BAC arrays, composed of 6500 RPCI-23 and RPCI-24 clones, represent ~0.4 Megabase resolution and are printed in triplicate on a single slide. These clones were chosen by virtue of their STS content and association with cancer.

Information is available on the Hartwell Center website located at http://www.hartwellcenter.org/bio_services/fungen/cDNA.php or contact Dr. Michael Wang (extension 4224, michael.wang@stjude.org).