Functional Genomics
 

> Clone Retrieval

Functional Genomic staff currently maintain some cDNA clone collections from mouse and human libraries corresponding to some arrays (chips) printed by the Functional Genomics lab.  After performing array experiments using these chips, you may obtain a bacterial culture of any clones of interest that you wish to investigate further.  We do not have clones for all arrays.  You can query HCNetDat to see if we have your clone(s) of interest (see below).  We recommend that you re-sequence any clones you receive to re-confirm clone identity.

How to Query and order available clones

  1. Clone queries can now be done using the Hartwell Center's DNA microarray gene annotation database, HCNetDat.  Here's how:
    • Click here to access HCNetDat.
    • Click "search arrays" and query HCNetDat using keywords, accession numbers, etc.  HCNetDat will display the corresponding list.
    • Click the "change view" button.  Click the checkboxes for "PlateName/Row/Column", "Clone ID" and "Vector" and click the "Change View" button at the bottom of the page.  If clones are available for your queried results, you will see information in these 3 columns.  If no information is shown in these columns, we do not have clones for those queried results.  Note that human clones have plate names beginning with "H" and mouse clones have plate names beginning with "M".
    • Select the desired clones by clicking the checkbox to the left of each line and click "Add To Genecart".  Click the "View Genecart" button.
    • Check the clones that you wish to order.
    • Export the gene cart and copy and paste into the request form below.
  2. Fill out the request form.  You may manually fill out the request form or use the instructions above to copy and paste from your HCNetDat query (see step 1 above).  To insure accurate retrieval of your specified clone, the CLONE ID FIELD (obtained from the downloaded clone list) must be completed. 
  3. Bring the completed request form to the Functional Genomics lab in the Hartwell center (room DT1008 in the Danny Thomas tower).
  4. Download the "Guide for Handling Clones" to obtain information about growth and PCR conditions, sequence information and more.

Request Form

Click here to download the request form (pdf or word doc). 

Quality Assurance

Most clone collections have been obtained from commercial vendors such as Incyte Genomics and have been sequence verified by the vendor.  Selected clones are re-sequence verified by the Functional Genomics lab.

Fees

Please follow this link to a general fees page.

Click here for the Guide for Handling of Clones

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